Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells
نویسندگان
چکیده
منابع مشابه
Telomerase Reverse Transcriptase Mediated Immortalization of Human Bone Marrow Stromal Cells
Primary human bone marrow stromal cells (hMSCs) were transfected with human telomerase reverse transcriptase (hTERT) gene with lipofection method. The hTERT transfected hMSCs of passage 100 underwent chondrogenesis induction with dexamethasone, transforming the growth factor β and vitamin C, osteogenesis induction with dexamethasone, β glycerophosphoric acid and vitamin C, and cardiomyocyte ind...
متن کاملImmortalization of human CD8+ T cell clones by ectopic expression of telomerase reverse transcriptase.
Replicative senescence of T cells is correlated with erosion of telomere ends. Telomerase plays a key role in maintaining telomere length. Therefore, it is thought that telomerase regulates the life span of T cells. To test this hypothesis, we have over-expressed human telomerase reverse transcriptase in human CD8(+) T cells. Ectopic expression of human telomerase reverse transcriptase led to i...
متن کاملHedgehog Signaling Regulates Telomerase Reverse Transcriptase in Human Cancer Cells
The Hedgehog (HH) signaling pathway is critical for normal embryonic development, tissue patterning and cell differentiation. Aberrant HH signaling is involved in multiple human cancers. HH signaling involves a multi-protein cascade activating the GLI proteins that transcriptionally regulate HH target genes. We have previously reported that HH signaling is essential for human colon cancer cell ...
متن کاملBone marrow stromal cells and their application in neural injuries
Background: This article reviews experimental and clinical studies in which neural injuries repaired with bone marrow stromal cells. History: Bone marrow contains two kinds of stem cells: hematopoietic and nonhematopoietic (stromal) stem cell. In vitro studies indicate that bone marrow stromal cells have the capacity of differentiation into other cells (such as neural cell) under treatment wit...
متن کاملtargeting human telomerase reverse transcriptase by a simple sirna expression cassette in hepg2 cells
background human telomerase reverse transcriptase (htert) has become an ideal target for development of anticancer therapy. small interfering rnas (sirnas) are very powerful reagents for gene silencing and show promise for cancer gene therapy. however, only a small number of sirnas have been demonstrated to be effective. for gene therapy targeting htert, it is essential to develop a robust syst...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2014
ISSN: 1516-8913
DOI: 10.1590/s1516-89132014000100006